THE PREVALENCE OF FUNGAL INFECTION IN MEDICINAL PLANTS AT NGHE AN HOSPITAL OF TRADITIONAL MEDICINE, 2019

Tran Quang Phuc1, Ho Van Thang2, Do Thi Thanh Thuy3
1 National Hospital of Malariology, Parasitology and Entomology
2 Nghe An Hospital of Traditional Medicine
3 198 Hospital

Main Article Content

Abstract

Objectives: Due to poor storage conditions, a large percentage of medicinal plants are infected
with fungi. Parasitic fungi living in herbal medicine produce toxic substances such as aflatoxin,
and ochratoxin, affecting the health of users. The study was conducted in the Nghe An Hospital of
Traditional Medicine in 2019 to determine the prevalence of fungal infection and fungal composition
in medicinal herbs.
Subjects and methods: Subjects of the study included medicinal plants preserved at the drugstore of
Nghe An Hospital of Traditional Medicine. The study employed a descriptive experimental research
method in Laboratory.
Results: The prevalence of fungal infection by direct KOH examination was 40.0%, of which
filamentous fungi accounted for 92.0%, and yeast only 8.0%. The prevalence of fungal infection by
culture in the Saboraud agar medium was 48.1%, of which the rate of fungal infection in Chinese
medicinal herbs was 41.1%, and in Vietnamese medicinal herbs was 62.2%. By PCR, 11 species
of fungi were identified, of which A. niger accounted for the highest percentage of 60.0% (30/50),
followed by A. tubingensis 18.0% (9/50).
Conclusions: The prevalence of fungal infection in medicinal plants was as high as 48.1% with a
diverse species composition (11 species). It is necessary to have appropriate preservation measures
to reduce fungal infection in medicinal plants.

Article Details

References

[1] Bộ Y tế, Ký sinh trùng thực hành, Sách đào tạo
dược sỹ đại học, NXB Y học Hà Nội, 283 Trang,
2012.
[2] Cornell University, USA, Aflatoxins: Ocucurrence
and Healt, Risks, Tekboo, 2013.
[3] David GS, Mycotoxins in Crops: A threat to
Human and Domestic Animal Health,Virgnia
Polytechnic Institute and State University
Blachsburg. VA., 2013.
[4] Nguyễn Đình Nga, Nguyễn Thị Kiều Khanh,
Văn Phố, “Khảo sát mức độ nhiễm nấm mốc và
aflatoxin trong một số dược liệu bán ở Quận 5 –
Thành phố Hồ Chí Minh”, Tạp chí Y học thành
phố Hồ Chí Minh, “Tập 16”, Phụ bản số 1, Trang
93-96, 201223
[5] World Health Organization, Jont FAO/WHO
Food Standards Programe Condex Committee On
Contannamnts In Foods, Food and Agriculture
Organization of the United Nations, 2013, pp.118.
[6] World Health Organization, Jont Fao/WHO Food
Standards Programe Condex Committee On
Contannamnts In Foods, Food and Agriculture
Organization of the United Nations, 2018, 178 pp
[7] National Institute of Malariology Parasitology
and Enmotology, Health Researth Methology,
Postgraduate and graduate textbooks, Medical
Publishing House, Hanoi, 2018.
[8] Lý Thu Hương, Mức độ nhiễm vi nấm sinh
ochratoxin A trong đất, và rễ cây cà phê ở một số
vùng trồng cà phê trọng điểm, Luận văn thạc sỹ
chuyên ngành Vi sinh vật, Đại học Khoa học tự
nhiên- Đại học Quốc gia Hà Nội, 2012.
[9] Đậu Huy Hoàn, Nghiên cứu thực trạng nhiễm
nấm và aflatoxin trên các vị thuốc đông dược tại
một số bệnh viện tỉnh Nghệ An (2016 -2018),
Luận án tiến sỹ y học, 2018.
[10] Andre EK, Ali A, Toufic R et al., , Defferentiation
Between Aspergillus flavus and A Aspergillus
parasiticus from pure culture and alflatoxinContaminated grapes using PCR-RFLP analysis
of aflr-aflj intergennic specer, Jounal of food
science, Vol.76(4), 2011, pp.247-253.
[11] Ajfand, Africa Scholarly Science Communications
Trust, African Jounal of Food Agricuture,
Nutrition and Development, Vol.16(3), 2016,
pp.10992-11003.
[12] Pereira M, Diversity and association of
filamentous fungi in coffee beans under organic
and conventional cultivation, African Jounal of
Microbiology Research, Vol.8(6), 2014, pp.2505-
2512, Doi: 10.5897/AJMR2013.6571.