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Published: 2025-04-15
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DOI: https://doi.org/10.52163/yhc.v66iCD4.2349
Issue
Vol. 66 No. CĐ4 NCKH
Section
Articles
How to Cite
Thiều, N. Q., Ngọc, N. T. H., Phượng, N. T., Bình, N. T. H., Hồng, N. V., & Giang, N. Đức. (2025). 31. STUDY ON Pvcrt GENE MUTATIONS ASSOCIATED WITH CHLOROQUINE RESISTANCE IN PLASMODIUM VIVAX IN MUONG TE, LAI CHAU USING real-time PCR AND WHOLE-GENOME SEQUENCING. Vietnam Journal of Community Medicine, 66(CĐ4 NCKH). https://doi.org/10.52163/yhc.v66iCD4.2349

31. STUDY ON Pvcrt GENE MUTATIONS ASSOCIATED WITH CHLOROQUINE RESISTANCE IN PLASMODIUM VIVAX IN MUONG TE, LAI CHAU USING real-time PCR AND WHOLE-GENOME SEQUENCING

Nguyen Quang Thieu1, Nguyen Thi Hong Ngoc1, Nguyen Thi Phuong2, Nguyen Thi Huong Binh1, Nguyen Van Hong1, Nguyen Duc Giang1
1 National Institute of Malariology, Parasitology and Entomology
2 Hanoi University of Public Health

Main Article Content

Abstract

Objective: This study investigates Pvcrt gene expression and mutations associated with Chloroquine resistance in Plasmodium vivax isolates from Muong Te district, Lai Chau province, Vietnam.


Methods: A cross-sectional study was conducted on 48 blood samples positive for P. vivax, collected from October 2022 to March 2024. Pvcrt expression was quantified by qPCR, and mutations were identified using whole genom Illumina sequencing. In addition, a literature review (2008-2023) was performed to evaluate Pvcrt roles globally.


Results: 14.6% of samples showed increased Pvcrt expression. C350T mutation was present in 4.2% and codon 10 insertions (K10) in 12.5%. C350T was significantly associated with overexpression (p < 0.05). Literature confirms Pvcrt involvement in Chloroquine resistance across Southeast and South Asia.


Conclusion: Pvcrt plays a potential role in Chloroquine resistance surveillance in P. vivax. Integration of molecular monitoring of Pvcrt into Vietnam’s malaria control strategies is recommended.

Article Details

Keywords

Plasmodium vivax, Pvcrt, Chloroquine resistance, mutation, gene expression, Vietnam

References

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