Article Sidebar

PDF (Tiếng Việt)
Published: 2023-07-28
Abstract Views: 167
Views PDF (Tiếng Việt): 23
DOI: https://doi.org/10.52163/yhc.v64i4.724
Issue
Vol. 64 No. 4
Section
Articles
How to Cite
Tiến, C. Đức ., Trang, N. T. T. ., Đạt, N. T. ., Trường, H. T. ., Chuyên, N. V. ., Ba, N. V. ., & Anh, L. T. . (2023). 2. OPTIMIZATION OF CONJUGATE RELEASE AND ANTIBODY CONCENTRATIONS IN THE CAPTURING ZONES TO ENHANCE THE SENSITIVITY OF LATERAL FLOW IMMUNOASSAY FOR DETECTION OF AFLATOXIN B 1. Vietnam Journal of Community Medicine, 64(4). https://doi.org/10.52163/yhc.v64i4.724

2. OPTIMIZATION OF CONJUGATE RELEASE AND ANTIBODY CONCENTRATIONS IN THE CAPTURING ZONES TO ENHANCE THE SENSITIVITY OF LATERAL FLOW IMMUNOASSAY FOR DETECTION OF AFLATOXIN B 1

Chu Duc Tien1, Nguyen Thi Thu Trang1, Nguyen Trong Dat2, Hoang Thi Truong1, Nguyen Van Chuyen1, Nguyen Van Ba1, Le Tuan Anh1
1 Vietnam Military Medicine University
2 Military Institute of Preventive Midicine

Main Article Content

Abstract

Objectives: Optimization of conjugate release and antibody concentrations in the capturing zones to enhance the sensitivity of lateral flow immunoassay for detection of Aflatoxin B1.


Methods: Optimal parameters include: the concentration of monoclonal antibodies spread in the capturing zones, the effects of different chemical buffers for pre-treatment conjugate pad.


Results: The concentration of the capturing antibody in the test line were 0,75 mg/ml. The results showed that the stability of the antibody and the conjugate release were highest when using borate buffer and sucrose.


Conclusions: We have enhanced the sensitivity of Lateral flow immunoassay by optimizing important parameters affecting detection limit and color signal intensity on test line.

Article Details

Keywords

Lateral flow immunoassay; LFIA; rapid detection; Aflatoxin B1; sensitivity.

References

[1] Galvano F, Ritieni A, Piva G et al., The
Mycotoxins Blue Book; Nottingham University
Press: Nottingham, UK, 2005; pp. 187–224
[2] Ammida NHS, Micheli L, Palleschi G,
Electrochemical immunosensor for determination
of AFB1 in barley. Anal. Chim. Acta 2004, 520,
159–164.
[3] Oshima H, Kinoshita M, Effects of sugars on
the thermal stability of a protein. J. Chem. Phys.
2013, 138, 245101.
[4] Yazdani Y, Mohammadi S, Yousefi M et al.,
Preliminary Assessment of Various Additives
on the Specific Reactivity of Anti-rHBsAg
Monoclonal Antibodies. Avicenna J. Med.
Biotechnol. 2015, 7, 145–150.
[5] Pan R, Jiang Y, Sun L et al., Gold nanoparticlebased
enhanced lateral flow immunoassay for
detection of Cronobacter sakazakii in powdered
infant formula. J Dairy Sci. 2018;101(5):3835–3843.
[6] Sangdae L, Performance Improvement of the
One-Dot Lateral Flow Immunoassay for AFB1
by Using a Smartphone-Based Reading System.
Sensors 2013, 13, 5109-5116.