56. RESEARCH ON THE LIVER PROTECTIVE EFFECT OF THE COMBINED EXTRACT FROM 50% ETHANOL EXTRACT OF ANDROTOGRAPHIS PANICULATA (Burm. f.) Nees. AND 50% ETHANOL EXTRACT OF ECLIPTA PROSTRATA (L.) L. ON THE ETHANOL-INDUCED LIVER DAMAGE MOUSE MODEL
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Abstract
Objective: This study aimed to investigate the hepatoprotective effect of the combination extract of Andrographis paniculata and Eclipta prostrata (XTL-CM extract) on a chronic ethanol-induced liver injury model in mice.
Subjects and Methods: ICR mice were administered ethanol orally for 4 weeks with gradually increasing concentrations each week (10%, 20%, 30%, and 40% ethanol). The animals were then treated with the XTL-CM extract at doses of 0.504 g/kg and 1.08 g/kg in the treatment groups, and silymarin at a dose of 0.1 g/kg in the reference group, for five weeks. Subsequently, the mice were evaluated for biochemical parameters, including AST and ALT levels, as well as oxidative stress-related hepatoprotective effects on hepatic MDA and GSH content.
Results: The combination extract from XTL-CM at doses of 0.504 g/kg and 1.08 g/kg significantly reduced serum AST levels (19.26% and 29.05%; respectively); ALT levels (24.53% and 23.70%; respectively) after 4 weeks; and hepatic MDA content (31.11% and 31.76%; respectively) after 5 weeks. Moreover, the extract increased hepatic GSH content (44.66% and 54.88%, respectively). These effects were comparable to those observed in the group treated with silymarin at 0.1 g/kg in the chronic ethanol-induced liver injury model.
Conclusion: The combination extract of Andrographis paniculata and Eclipta prostrata demonstrated hepatoprotective activity against chronic ethanol-induced liver injury through antioxidant mechanisms, similar to the effect of silymarin at 0.1 g/kg.
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Keywords
Andrographis paniculata, Eclipta prostrata, hepatoprotective effect, ethanol, AST, ALT, MDA, GSH.
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