Article Sidebar

PDF (Tiếng Việt)
Published: 2025-07-01
Abstract Views: 8
Views PDF (Tiếng Việt): 10
DOI: https://doi.org/10.52163/yhc.v66ienglish.2771
Issue
Tập 66 VoL.English Version
Section
Articles
How to Cite
Anh, V. H., Nam, N. G., Hanh, N. T. H., Kien, N. T., & Hung, T. V. (2025). 28. PRELIMINARY EVALUATION OF THE DIAGNOSTIC VALUE OF HFLC IN DIFFERENTIATING DENGUE FEVER FROM OTHER FEBRILE ILLNESSES. Vietnam Journal of Community Medicine, 66(english). https://doi.org/10.52163/yhc.v66ienglish.2771

28. PRELIMINARY EVALUATION OF THE DIAGNOSTIC VALUE OF HFLC IN DIFFERENTIATING DENGUE FEVER FROM OTHER FEBRILE ILLNESSES

Vu Hong Anh1, Nguyen Giang Nam1, Nguyen Thi Hien Hanh1, Nguyen Trung Kien1, Ta Viet Hung1
1 103 Military Hospital

Main Article Content

Abstract

Abstract: Dengue fever (DF) remains a primary global health concern. High Fluorescence Lymphocyte Count (HFLC) reflects the immune response to infection by quantifying antibody-secreting cells (ASC), such as plasmablasts/plasma cells. ASCs are known to increase markedly in dengue compared to other febrile illnesses (OFIs).


Objective: This study aimed to preliminarily evaluate the diagnostic value of HFLC in distinguishing dengue fever from other febrile illnesses.


Methods: A cross-sectional study was conducted on 317 patients presenting with fever on admission. Clinical features and laboratory parameters, including HFLC# and HFLC%, were collected. Patients were categorized into the dengue infection (DI) group (n = 267) and the OFI group (n = 50).


Results: The DI group had significantly higher median HFLC# (0.11 G/L) and HFLC% (5.2%) compared to the OFI group (0.01 G/L and 0.20%, respectively; p < 0.001). ROC analysis showed good diagnostic performance, with AUCs of 0.794 (HFLC#) and 0.842 (HFLC%). A cut-off value of 0.025 G/L for HFLC# yielded 70.4% sensitivity and 78.0% specificity. For HFLC%, a threshold of 0.75% resulted in 70.8% sensitivity and 88.0% specificity.


Conclusion: HFLC demonstrates potential as a rapid and accessible marker for differentiating dengue fever from other febrile illnesses in clinical practice.

Article Details

Keywords

Dengue fever, HFLC, diagnosis.

References

[1] Whitehorn, J. & Farrar, J. Dengue. Br. Med. Bull. 95, 161–173 (2010). doi:10.1093/bmb/ldq019.
[2] Ha, V. H. ., Ha, T. T. T. ., Thai, P. Q. ., Nghia, N. D. ., Nam, V. S. ., & Duong, T. N. . (2022). Sốt xuất huyết dengue tại Việt Nam giai đoạn 1999 – 2020. Vietnam J. Prev. Med. 32, 9–15 (2022). doi:https://doi.org/10.51403/0868-2836/2022/603.
[3] Kularatne, S. A. M. Dengue fever. BMJ 351, h4661 (2015). doi:10.1136/bmj.h4661.
[4] Oehadian, A. et al. New parameters available on Sysmex XE-5000 hematology analyzers contribute to differentiating dengue from leptospirosis and enteric fever. Int. J. Lab. Hematol. 37, 861–868 (2015). doi:10.1111/ijlh.12422.
[5] Low, J. G. H. et al. The Early Clinical Features of Dengue in Adults: Challenges for Early Clinical Diagnosis. PLoS Neglected Tropical Diseases vol. 5 (2011). doi:10.1371/journal.pntd.0001191.
[6] Chen, L. H. & Wilson, M. E. Dengue and chikungunya infections in travelers. Curr. Opin. Infect. Dis. 23, 438–444 (2010). doi:10.1097/QCO.0b013e32833c1d16.
[7] Linssen, J. et al. Identification and quantification of high fluorescence-stained lymphocytes as antibody-synthesizing/secreting cells using the automated routine hematology analyzer XE-2100. Cytometry B. Clin. Cytom. 72, 157–166 (2007). doi:10.1002/cyto.b.20150.
[8] Wrammert, J. et al. Rapid and massive virus-specific plasmablast responses during acute dengue virus infection in humans. J. Virol. 86, 2911–2918 (2012). doi:10.1128/JVI.06075-11.
[9] Lee, H. et al. Comparison of Six Commercial Diagnostic Tests for the Detection of Dengue Virus Non-Structural-1 Antigen and IgM/IgG Antibodies. Ann. Lab. Med. 39, 566–571 (2019). doi:10.3343/alm.2019.39.6.566.