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Published: 2025-05-20
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DOI: https://doi.org/10.52163/yhc.v66iCD8.2573
Issue
Vol. 66 No. CĐ8-NCKH
Section
Articles
How to Cite
Mến, C. V., Hoàng, P. H., Chuyên, N. V., Ba, N. V., Châu, N. A. B., Minh, Đỗ Q., Nhân, N. Đình, & Nhi, N. H. Y. (2025). 27. DEVELOPMENT AND VALIDATION OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE QUANTIFICATION OF AFLATOXIN B1 IN HERBAL DRUGS AND CEREALS. Vietnam Journal of Community Medicine, 66(CĐ8-NCKH). https://doi.org/10.52163/yhc.v66iCD8.2573

27. DEVELOPMENT AND VALIDATION OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE QUANTIFICATION OF AFLATOXIN B1 IN HERBAL DRUGS AND CEREALS

Chu Van Men1, Pham Huy Hoang1, Nguyen Van Chuyen1, Nguyen Van Ba1, Nguyen An Bao Chau2, Do Quang Minh3, Ngo Dinh Nhan4, Ngo Hoang Yen Nhi4
1 Vietnam Military Medical University
2 Vinschool Smart City Secondary and High School
3 Le Loi High School
4 Drug Administration, Ministry of Health

Main Article Content

Abstract

Objectives: A high-performance liquid chromatography method was developed and validated for the quantification of Aflatoxin B1 in Herbs and cereals.


Method: The proposed method was validated according to ICH guidelines for specificity, repeatability, linearity, accuracy, and precision.


Results: The method was achieved including Waters C18 column (50 mm × 2.1 mm; 1.7 µm), the mobile phase contained aqueous 0.1% phosphoric acid:acetonitrile 0.2% (33:67) in isocratic with flow rate of 0.2 ml/min, injection volume of 20 μl, and detector PDA-UV at 354 nm. The developed method showed the present of Aflatoxin B1 in 2 Herbs and cereals samples, account for about 2% of samples analyzed.


Conclusion: The study was successfully developed and validated high-performance liquid chromatography method for quantification of Aflatoxin B1 in Herbs and cereals. This is the background for further studies.

Article Details

Keywords

Herbal drugs, cereals, Aflatoxin B1, high-performance liquid chromatography.

References

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