46. STUDY ON ACUTE TOXICITY AND ANTI-INFLAMMATORY EFFECTS OF EUDIA LEPTA STEM EXTRACT IN MICE
Main Article Content
Abstract
Objectives: Supplement the scientific evidence on the safety profile and therapeutic potential of the Euodia lepta stem extract in aiding the treatment of inflammation, through acute toxicity evaluations and in vivo assessments of anti-inflammatory activity in mice.
Subjects and methods: Powder of Euodia lepta stem (2 kg) was extracted by the percolation method with 96% ethanol and concentrated under reduced pressure to obtain 0.108 kg of crude extract, achieving an extraction efficiency of 5.04%. The chemical composition of alkaloids, flavonoids, coumarins and saponins is determined by characteristic chemical reactions. The main active alkaloid (dictamnine, evolitrine, and kokusaginine) in the extract was determined by thin layer chromatography. The acute oral toxicity of the extract was determined by the Behrens method in mice. An in vivo anti-inflammatory test was conducted using a carrageenan-induced paw edema model in mice, and the degree of paw swelling was measured to evaluate the anti-inflammatory efficacy of the extract.
Results: The chemical composition of alkaloids, flavonoids, coumarin and saponin were identified. The main alkaloid constituents in the extract were performed using thin-layer chromatography, which corresponded to those of the standard reference alkaloids dictamnine, evolitrine, and kokusaginine. The inability to determine the LD50 of the Euodia lepta stem extract when administered orally to mice at a maximum dose of 35 g/kg body weight suggests that the extract has a relatively high safety profile, as no signs of lethality were observed even at such a high dose. The Euodia lepta stem extract at a dose of 100 mg/kg and 200 mg/kg significantly reduced paw edema in mice, with this effect showing a statistically significant difference compared to the control group (p < 0.01). Furthermore, the extract demonstrated equivalent effectiveness in reducing paw edema compared to the positive control group treated with diclofenac (5 mg/kg) at 3 hours after carrageenan sub-plantar injection (p < 0.01) and superior efficacy at 24 hours after (p < 0.001).
Conclusion: The Euodia lepta stem extract is not only safe but also demonstrates a significant effect in reducing paw edema compared to conventional anti-inflammatory drugs. The stem of Euodia lepta may have the potential to develop into an effective anti-inflammatory medicinal herb.
Article Details
Keywords
Euodia lepta stem, Melicope ptelefolia, alkaloid, acute toxicity, anti-inflammatory
References
[2] Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J et al, Inflammatory Responses and Inflammation-Associated Diseases in Organs, Oncotarget, 2018, 9, 7204-7218.
[3] Bagad A.S, Joseph J.A, Bhaskaran N, Agarwal A, Comparative Evaluation of Anti-Inflammatory Activity of Curcuminoids, Turmerones and Aqueous Extract of Curcuma longa, Advances in Pharmacological and Pharmaceutical Sciences, 2013, 805756.
[4] Camargo A, Rangel-Zuñiga O.A, Haro C et al, Olive oil phenolic compounds decrease the postprandial inflammatory response by reducing postprandial plasma lipopolysaccharide levels, Food Chemistry, 2014, 162, 161-71.
[5] Nikniaz Z, Ostadrahimi A, Mahdavi R et al, Effects of Elaeagnus angustifolia L. supplementation on serum levels of inflammatory cytokines and matrix metalloproteinases in females with knee osteoarthritis, Complementary Therapies in Medicine, 2014, 22 (5), 864-9.
[6] Huỳnh Minh Đạo, Nguyễn Thị Ngọc Bích, Nguyễn Thị Thúy Liễu, Trịnh Thị Quỳnh, Nghiên cứu đặc điểm thực vật và thành phần hóa học cây ba chạc (Euodia lepta (Spreng.) Merr, họ cam (Rutaceae)) thu hái tại Đà Nẵng, Tạp chí Y Dược học Cần Thơ, 2023, 58, 122-130.
[7] Lee B.W, Quy Ha T.K, Park E.J, Cho H.M, Ryu B, Doan T.P, Lee H.J, Oh W.K, Melicopteline A-E, Unusual Cyclopeptide Alkaloids with Antiviral Activity against Influenza A Virus from Melicope pteleifolia, J Org Chem, 2021 Jan 15, 86 (2), 1437-1447.
[8] Mahani Mahadi et al, The potential effects of Melicope ptelefolia root extract as an anti-nociceptive and anti-inflammatory on animal models, Bulletin of Faculty of Pharmacy, Cairo University, 2016, 54 (2), 237-241.
[9] Yao Q, Gao Y, Lai C, Wu C, Zhao C.L, Wu J.L, Tang D.X, The phytochemistry, pharmacology and applications of Melicope pteleifolia: A review, The Journal of Ethnopharmacology, 2020, 6, 251, 112546.
[10] Bộ Y tế, Dược Điển Việt Nam V, Nhà xuất bản Y học, 2018.
[11] Đỗ Tất Lợi, Những cây thuốc và vị thuốc Việt Nam, Nhà xuất bản Thời Đại, 2016, 123.
[12] Nguyễn Thị Hồng Ngọc, Nguyễn Diệp Huy Phong, Hồ Thanh Phát, Nguyễn Hoàng Tính, Bùi Thảo Nhi, Nguyễn Văn Luân, Đỗ Châu Minh Vĩnh Thọ, Ngiên cứu tác dụng kháng viêm của cao chuẩn hóa kiểm soát hàm lượng các curcumin từ thân rễ nghệ vàng (Rhizoma Curcuma longa L.) trên chuột nhắt trắng, Tạp chí Y học Việt Nam, 2023, 530 (1B).
[13] Lai W, Tan Y, Yang W, Zhang J, Liu M, Study on the acute toxicity of the aqueous extract of Polygonum hydropiper, Ficus pumila and Evodia lepta, Journal of Hainan Medical University, 2010, 16, 411-412.
[14] Sulaiman M.R, Mohd Padzil A, Shaari K, Khalid S, Shaik Mossadeq W.M, Mohamad A.S, Ahmad S, Akira A, Israf D, Lajis N, Antinociceptive activity of Melicope ptelefolia ethanolic extract in experimental animals, Journal of Biomedicine and Biotechnology, 2010, 937642.