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Published: 2021-12-09
Abstract Views: 352
DOI: https://doi.org/10.52163/yhc.v62i7%20(2021).214
Issue
Vol. 62 No. 7 (2021)
Section
Articles
How to Cite
Loan, N. T. ., Linh, N. T. ., Hiền, N. M. ., & Huyền, L. T. . (2021). USE MULTIPLEX REALTIME PCR METHODS FOR RAPID DETECTION OF FIVE BACTERIAL PATHOGENS CAUSING NOSOCOMIAL INFECTION: STAPHYLOCCCUS AUREUS, ESCHERICHIA COLI, PSEUDOMONAS AERUGINOSA, KLEBSIELLA PNEUMONIAE, ACINETOBACTER BAUMANNII ISOLATED FROM RESPIRATORY SAMPL. Vietnam Journal of Community Medicine, 62(7 (2021). https://doi.org/10.52163/yhc.v62i7 (2021).214

USE MULTIPLEX REALTIME PCR METHODS FOR RAPID DETECTION OF FIVE BACTERIAL PATHOGENS CAUSING NOSOCOMIAL INFECTION: STAPHYLOCCCUS AUREUS, ESCHERICHIA COLI, PSEUDOMONAS AERUGINOSA, KLEBSIELLA PNEUMONIAE, ACINETOBACTER BAUMANNII ISOLATED FROM RESPIRATORY SAMPL

Nguyen Thi Loan1, Nguyen Trong Linh2, Nguyen Minh Hien3, La Thi Huyen2
1 Graduate University of Science and Technology, Vietnam Academy of Science and Technology
2 Insitute of Biotechnology, Vietnam Academy of Science and Technology
3 Thanh Nhan Hospital

Main Article Content

Abstract

Purposes: Using the multiplex realtime PCR assay to simultaneously detect five nosocomial pathogens: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetorbacter bauminnii isolated from respiratory specimens. Objectives and Methods: 47 respiratory specimens from patients were treated at Thanh Nhan and Duc Giang hospitals as nosocomial infection. The patient samples were divided into 2 parts, one part was used for culture test and the other part was performed by multiplex real-time PCR assay. Results: 40/47 samples were positive (85.1%), the multiplex real-time PCR method can simultaneously detect 5 nosocomial pathogens from respiratory specimens, similarity coefficient with the culture method is 0.97 (p<0.05).

Article Details

Keywords

Respiratory specimens, Multiplex real-time PCR, nosocomial infection, nosocomial pathogens.

References

1. European Centrer for Disease Prevention and Control, Antimicrobial resistance surveillance in Europe 2015. Annual Report of the European Antimicrobial Resistance Surveillance Network (EARS-Net). Stockholm: ECDC, 2017.
Available from: http://ecdc.europa.eu/en/publications/Publications/ antimicrobial-resistance-europe-2015.pdf

2. European Centre of disease Prevention and Control, Health care- associated infections acquited in intensive care units. Annual epidemiological report for 2016. Stockholm. ECDC, 2018.

3. Shelley SM, Erin OL, Sarah JJ et al., Changes in Prevalence of Health Care–Associated Infections in U.S. Hospitals. N Engl J Med; 2018, 379:1732 -1744. DOI: 10.1056/NEJMoa1801550.

4. Trung DV, Research on antibiotic resistance of Staphylococcus aureus and Escherichia coli at 108 Central Military Hospital from April to June 2016”, Clinical Medicine 108, 2017; 5:157-161.

5. Ngoc NH, Characteristics of bacteria causing nosocomial infections at the Intensive Care Unit of Phu Tho General Hospital in 2016”, Vietnamese Medicine, 2017; 1:73-78.

6. Yang Q, Rui Y, Two Multiplex Real-Time PCR Assays to Detect andDifferentiate Acinetobacter baumannii and Non- baumannii Acinetobacter spp. Carrying blaNDM, blaOXA-23-Like, blaOXA-40-Like, blaOXA-51-Like, and blaOXA-58-Like Genes. PLoS One, 11(7): p. e0158958, 201.

7. Wang HY, Comparison of multiplex real-time PCR and PCR-reverse blot hybridization assay for the direct and rapid detection of bacteria and antibiotic resistance determinants in positive culture bottles. J Med Microbiol, 2016; 65(9): 962-74.