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Published: 2026-07-06
Abstract Views: 15
DOI: https://doi.org/10.52163/yhc.v67iCD8.5690
Issue
Vol. 67 No. CĐ8-NCKH
Section
Articles
How to Cite
Thành, L. C., Ánh, N. N., Tiệp, N. K., & Thiện, N. Đức. (2026). INVESTIGATION OF THE ANTIMICROBIAL ACTIVITY AND CANDIDA ALBICANS KILL-KINETICS OF 0.12% CHLORHEXIDINE DIGLUCONATE MOUTHWASH IN DENTISTRY. Vietnam Journal of Community Medicine, 67(CĐ8-NCKH). https://doi.org/10.52163/yhc.v67iCD8.5690

INVESTIGATION OF THE ANTIMICROBIAL ACTIVITY AND CANDIDA ALBICANS KILL-KINETICS OF 0.12% CHLORHEXIDINE DIGLUCONATE MOUTHWASH IN DENTISTRY

Ly Công Thanh1, Nguyen Ngoc Anh1, Nguyen Khac Tiep1, Nguyen Duc Thien1
1 Hanoi University of Pharmacy

Main Article Content

Abstract

Objective: The study aimed to evaluate the broad-spectrum antimicrobial activity and killing kinetics of a mouthwash containing 0.12% Chlorhexidine Digluconate (CHX-D) combined with 3% PEG4000.


Research methods: Antimicrobial activity was determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against 4 strains: E.coli, S.aureus, S.mutans, and C.albicans. Concentration-dependent effects (1-16 × MIC) were assessed for all strains, while time-kill kinetics (10-60 seconds) and microbial density (10⁵-10⁶ CFU/ml) were evaluated for C.albicans.


Results: Mouthwash CHX-D 0.12% showed strong activity with MBC/MIC ≤ 2 for bacteria and ≤ 4 for C.albicans. The solution eliminated > 99.9% of C.albicans after 10 seconds at 10⁵ CFU/ml and reached 99.99% after 40 seconds; at 10⁶ CFU/ml, it still achieved 99% after 10 seconds and complete killing after 60 seconds.


Kết luận: Kết quả khẳng định nước súc miệng CHX-D 0,12% là chế phẩm kháng khuẩn phổ rộng, diệt vi sinh vật nhanh và hiệu quả.

Article Details

Keywords

Chlorhexidine digluconate, antimicrobial, C.albicans, mouthwash, MIC/MBC.

References

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[5] Nguyễn Khắc Tiệp, Nguyễn Ngọc Ánh, Nguyễn Đức Thiện. Khảo sát độc tính với màng tế bào hồng cầu của dung dịch Chlorhexidine digluconate và dung dịch nước súc miệng Chlorhexidine diluconate 1200 µg/mL. Tạp chí Khoa học và Công nghệ Đại học Thái nguyên, 2025, 230, (05): 346-352. doi: 10.34238/tnu-jst.10947.