Bài báo khoa học Tập 66 Số VoL 66. English Version No 1, 2025 15/08/2025

28. PRELIMINARY EVALUATION OF THE DIAGNOSTIC VALUE OF HFLC IN DIFFERENTIATING DENGUE FEVER FROM OTHER FEBRILE ILLNESSES

Vu Hong Anh1, Nguyen Giang Nam1, Nguyen Thi Hien Hanh1, Nguyen Trung Kien1, Ta Viet Hung1
1 103 Military Hospital
Tác giả liên hệ: Bs.vuhonganh@gmail.com
DOI: 10.52163/yhc.v66ienglish.2771
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Từ khóa
Dengue fever HFLC diagnosis.
Tóm tắt

Abstract: Dengue fever (DF) remains a primary global health concern. High Fluorescence Lymphocyte Count (HFLC) reflects the immune response to infection by quantifying antibody-secreting cells (ASC), such as plasmablasts/plasma cells. ASCs are known to increase markedly in dengue compared to other febrile illnesses (OFIs).

Objective: This study aimed to preliminarily evaluate the diagnostic value of HFLC in distinguishing dengue fever from other febrile illnesses.

Methods: A cross-sectional study was conducted on 317 patients presenting with fever on admission. Clinical features and laboratory parameters, including HFLC# and HFLC%, were collected. Patients were categorized into the dengue infection (DI) group (n = 267) and the OFI group (n = 50).

Results: The DI group had significantly higher median HFLC# (0.11 G/L) and HFLC% (5.2%) compared to the OFI group (0.01 G/L and 0.20%, respectively; p < 0.001). ROC analysis showed good diagnostic performance, with AUCs of 0.794 (HFLC#) and 0.842 (HFLC%). A cut-off value of 0.025 G/L for HFLC# yielded 70.4% sensitivity and 78.0% specificity. For HFLC%, a threshold of 0.75% resulted in 70.8% sensitivity and 88.0% specificity.

Conclusion: HFLC demonstrates potential as a rapid and accessible marker for differentiating dengue fever from other febrile illnesses in clinical practice.

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