Articles Vol. 65 No. 2 08/03/2024

24. STUDY ON BLOOD URIC ACID-LOWERING EFFECTS AND TOXICITY OF DRIED LOLOT LEAVE EXCITEMENT ON EXPERIMENTAL ANIMALS

Duong Anh Tuyet1,2, Ngo Nguyen Quynh Anh1,2, Vu Thi Minh Thu1,2, Nguyen Thi Thu Huyen1,2, Pham Thi Hang1,2, Nguyen Thi Dong3,4, Nguyen Thuy Duong5,6
1 Hai Duong Central College of Pharmacy
2 Trường Cao đẳng Dược Trung ương Hải Dương
3 Department of Science, Technology and Training, Ministry of Health
4 Cục Khoa học Công nghệ và Đào tạo, Bộ Y tế
5 Hanoi University of Pharmacy
6 Trường Đại học Dược Hà Nội
Corresponding author: qanhk63hup@gmail.com
DOI: 10.52163/yhc.v65i2.943
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Keywords
Gout; dried extract of lolot leaves; lowering uric acid; toxicity.
Abstract

Objective: The research aimed to investigate the impact of reducing blood uric acid and the toxicity of dried lolot leaf extract. The specific goals were to assess the efficacy of lowering blood uric acid and evaluate the acute and semi-chronic toxicity of the extract.

Research subjects and methods: The study involved evaluating the uric acid-lowering effect of dried lolot leaf extract using a model inducing acute hyperuricemia by injecting potassium oxonate through the peritoneum in white mice. Additionally, acute toxicity tests were conducted on white mice over a 14-day period, and semi-chronic toxicity tests were performed on rats over 28 days.

Results: The dried lolot leaf extract, administered at a dose of 300 mg/kg, demonstrated effectiveness in lowering blood uric acid, showing a reduction rate of 36.3% (p<0,05) compared to the control group. Importantly, the dried lolot leaf extract exhibited no acute toxicity at the tested doses, as evidenced by the absence of fatalities in the test batches, preventing the determination of LD50. Furthermore, in semi-chronic toxicity tests on mice subjected to repeated doses (330 mg/kg/day and 990 mg/kg/day) for 28 days, the extract did not display toxicity across parameters assessing general condition, liver function, kidney function, and hematopoietic function.

Conclusion: The dried lolot leaf extract was found to effectively lower uric acid without exhibiting signs of toxicity in the experimental animals.

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