Articles Vol. 65 No. 2 08/03/2024

17. STUDY ON PREPARATION OF NGL POWDERED EXTRACT BASED ON NHAT GAN LINH TRADITIONAL MEDICINAL FORMULATION

Pham Xuan Phong1,2, Chu Van Men3,4, Nguyen Duc Thanh5,6, Tran Thi Tuyet Nhung1,2, Bui Thi Bich Van3,4
1 Military Institute of Traditional Medicine
2 Viện Y học cổ truyền Quân đội
3 Vietnam Military Medical University
4 Học viện Quân y
5 Military Institute of Radiation Medicine and Cancer
6 Viện Y học phóng xạ và U bướu Quân đội, Cục Quân y
Corresponding author: chuvanmen@gmail.com
DOI: 10.52163/yhc.v65i2.935
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Keywords
Powdered extract, Nhat gan linh, spray-drying.
Abstract

Objectives: To develop the preparation process and quality assessment of the “NGL” powdered extract which originates from the “Nhat gan linh” traditional medicine.

Subjects and methods: Preparation of “NGL” powdered extract (including 15 medicinal materials: Radix Glehniae, Radix Rehmanniae glutinosae, Radix Angelicae sinensis, Fructus Meliae toosendan, Polyporus, Poria, Rhizoma Alismatis, Styli et stigmata Maydis, Cortex Phellodendri, Cortex Radicis Paeoniae suffruticosae, Fructus Corni officinalis, Fructus aurantii immaturus, Radix Bupleuri chinensis, Radix Eurycomae longifoliae, Radix Paeoniae lactiflorae) via hot extraction combined and spray-drying technique. The quality assessment of powdered extract included morphology, density index, compression index, loss on drying.

Results: Liquid extract NGL 3:1 was prepared using extract solvent as water, material; solvent 1:6 g/mL, extraction temperature 700C, extraction time 60 minutes, repeated 2 times. The spray-drying parameters were determined: mixure of spray-drying excipients maltodextrin:aerosil (30:70 w/w), excipients: extract solid 3:7 w/w; percentage of solid in spray solvent 15%, spray-drying temperature 1400C, speed of feed 30 ml/min; pump pressure 0.2 Mpa. The spray-drying yield was 91.80%; recovery yield of saikosaponin A and berberin was 92.54% and 86.38%, respectively. The quality parameters of NGL powdered extract included: dried and separated powder, loss on drying less than 5%; CI 32.82 ± 0,21%; content of saikosaponin A and berberin were 5.27 ± 0.02 and 93.33 ± 0.03 (mg/g), respectively.

Conclusions: The preparation process of the “NGL” powder was established using the spray-drying technique. The spray-drying parameters in preparation process of the “NGL” powder were also investigated. The powder quality was tested. This study contributed to a scientific base for further in-depth studies in order to prepare other dosage forms.

References
[1]
Đào Văn Long, Xơ gan, Bệnh học Nội khoa, Tập Google Scholar
[2]
, Nhà xuất bản Y học, 2012, 9-16. Google Scholar
[3]
Nguyễn Khánh Trạch, Phạm Thị Thu Hồ, Chẩn Google Scholar
[4]
đoán và điều trị xơ gan, Bài giảng bệnh học nội Google Scholar
[5]
khoa, Tập 2, 2015, 193-202.134 Google Scholar
[6]
Sung H, Ferlay J, Siegel RL et al., Global Google Scholar
[7]
Cancer Statistics 2020: GLOBOCAN Estimates Google Scholar
[8]
of Incidence and Mortality Worldwide for 36 Google Scholar
[9]
Cancers in 185 Countries. CA: a cancer journal Google Scholar
[10]
for clinicians, 71(3), 2021, 209-49. Google Scholar
[11]
Sollohub K, Cal K, Spray drying technique: Google Scholar
[12]
II. Current applications in pharmaceutical Google Scholar
[13]
technology, Journal of pharmaceutical sciences, Google Scholar
[14]
(2), 2010, 587-97. Google Scholar
[15]
Zhu YL, Li SL, Jin JL et al., Simultaneous Google Scholar
[16]
determination of six components of Danzhi Google Scholar
[17]
Xiaoyao Pill in beagle plasma by HPLC-MS/MS Google Scholar
[18]
and a study of pharmacokinetic of paeoniflorin Google Scholar
[19]
and geniposide after single-dose administration, Google Scholar
[20]
Journal of pharmaceutical and biomedical Google Scholar
[21]
analysis, 186, 113269, 2020. Google Scholar
[22]
Neag MA, Mocan A, Echeverría J et al., Google Scholar
[23]
Berberine: Botanical Occurrence, Traditional Google Scholar
[24]
Uses, Extraction Methods, and Relevance in Google Scholar
[25]
Cardiovascular, Metabolic, Hepatic, and Renal Google Scholar
[26]
Disorders, Frontiers in pharmacology, 9, 557, Google Scholar
[27]
Eom HY, Park SY, Kim MK et al., Comparison Google Scholar
[28]
between evaporative light scattering detection Google Scholar
[29]
and charged aerosol detection for the analysis Google Scholar
[30]
of saikosaponins, Journal of chromatography A, Google Scholar
[31]
(26), 2010, 4347-54. Google Scholar