Articles Vol. 64 No. 4 28/07/2023

2. OPTIMIZATION OF CONJUGATE RELEASE AND ANTIBODY CONCENTRATIONS IN THE CAPTURING ZONES TO ENHANCE THE SENSITIVITY OF LATERAL FLOW IMMUNOASSAY FOR DETECTION OF AFLATOXIN B 1

Tien Chu Duc1,2, Trang Nguyen Thi Thu1,2, Dat Nguyen Trong3,4, Truong Hoang Thi1,2, Chuyen Nguyen Van1,2, Ba Nguyen Van1,2, Anh Le Tuan1,2
1 Vietnam Military Medicine University
2 Học viện Quân y
3 Military Institute of Preventive Midicine
4 Viện Y học Dự phòng Quân đội
Corresponding author: ductien280190@gmail.com
DOI: 10.52163/yhc.v64i4.724
68 Views
34 Downloads
Abstract

Objectives: Optimization of conjugate release and antibody concentrations in the capturing zones to enhance the sensitivity of lateral flow immunoassay for detection of Aflatoxin B1.

Methods: Optimal parameters include: the concentration of monoclonal antibodies spread in the capturing zones, the effects of different chemical buffers for pre-treatment conjugate pad.

Results: The concentration of the capturing antibody in the test line were 0,75 mg/ml. The results showed that the stability of the antibody and the conjugate release were highest when using borate buffer and sucrose.

Conclusions: We have enhanced the sensitivity of Lateral flow immunoassay by optimizing important parameters affecting detection limit and color signal intensity on test line.

Keywords
Lateral flow immunoassay; LFIA; rapid detection; Aflatoxin B1; sensitivity.
References
[1]
Galvano F, Ritieni A, Piva G et al., The Google Scholar
[2]
Mycotoxins Blue Book; Nottingham University Google Scholar
[3]
Press: Nottingham, UK, 2005; pp. 187–224 Google Scholar
[4]
Ammida NHS, Micheli L, Palleschi G, Google Scholar
[5]
Electrochemical immunosensor for determination Google Scholar
[6]
of AFB1 in barley. Anal. Chim. Acta 2004, 520, Google Scholar
[7]
–164. Google Scholar
[8]
Oshima H, Kinoshita M, Effects of sugars on Google Scholar
[9]
the thermal stability of a protein. J. Chem. Phys. Google Scholar
[10]
, 138, 245101. Google Scholar
[11]
Yazdani Y, Mohammadi S, Yousefi M et al., Google Scholar
[12]
Preliminary Assessment of Various Additives Google Scholar
[13]
on the Specific Reactivity of Anti-rHBsAg Google Scholar
[14]
Monoclonal Antibodies. Avicenna J. Med. Google Scholar
[15]
Biotechnol. 2015, 7, 145–150. Google Scholar
[16]
Pan R, Jiang Y, Sun L et al., Gold nanoparticlebased Google Scholar
[17]
enhanced lateral flow immunoassay for Google Scholar
[18]
detection of Cronobacter sakazakii in powdered Google Scholar
[19]
infant formula. J Dairy Sci. 2018;101(5):3835–3843. Google Scholar
[20]
Sangdae L, Performance Improvement of the Google Scholar
[21]
One-Dot Lateral Flow Immunoassay for AFB1 Google Scholar
[22]
by Using a Smartphone-Based Reading System. Google Scholar
[23]
Sensors 2013, 13, 5109-5116. Google Scholar