Objective: To evaluate the results of newborn screening for G6PD enzyme deficiency using heel-prick blood samples at the Department of Laboratory Medicine, Thanh Hoa Obstetrics and Gynecology Hospital.
Subjects and methods: A retrospective cross-sectional descriptive study was conducted on 3328 newborns screened for G6PD deficiency using dried blood spot samples from September 2025 to March 2026. Screening was performed using the Victor2D fluorometric reader and the quantitative G6PD assay kit manufactured by Revvity. Parameters analyzed included the proportion of newborns at high risk, the rate of confirmed G6PD deficiency cases, and indicators related to the implementation of the screening process.
Results: The proportion of newborns with high-risk screening results for G6PD deficiency was 1.47%. Among the 49 newborns identified as high risk, 36 underwent confirmatory testing. The concordance rate between confirmatory testing and screening results was 97.22% (35/36) with male newborns accounting for a markedly higher proportion than female newborns.
Conclusion: Fluorometric screening for G6PD deficiency using neonatal heel-prick blood samples demonstrated feasibility and suitability for routine implementation in laboratory settings. This method contributes to the early detection of suspected G6PD deficiency cases in newborns, facilitating timely monitoring and intervention.