Articles Tập 67 Số CĐ9-Hội Y học Giới tính 29/07/2026

TESTICULAR HISTOLOGICAL CHARACTERISTICS FOLLOWING ADMINISTRATION OF THE HERBAL FORMULA HOA DAM SINH TINH DECOCTION IN AN EXPERIMENTAL MODEL

Quach Thi Yen1,2, Pham Thi Thuy An3,4, Doan Minh Thuy1,2, Nguyen Huyen Trang1,2, Pham Thi Diu1,2, Bui Thi Quynh1,2
1 Vietnam University of Traditional Medicine
2 Học viện Y Dược học Cổ truyền Việt Nam
3 VNU University of Medicine and Pharmacy
4 Trường Đại học Y dược, Đại học Quốc gia Hà Nội
DOI: 10.52163/yhc.v67iCD9.5814
32 Views
0 Downloads
Keywords
Hoa dam sinh tinh decoction, testicular structure, seminiferous tubules.
Abstract

Objective: To describe the microscopic characteristics of testicular tissue following the administration of the herbal formula Hoa dam sinh tinh decoction in an experimental model.

Subjects and methods: Male white rats with induced testicular dysfunction were administered the study drug. Post-treatment testicular samples were microscopically evaluated for the following parameters: testicular weight, seminiferous tubule diameter, germinal epithelium thickness, Johnsen score, germ cell count, and testicular interstitium.

Results: The seminiferous tubule diameter, germinal epithelium height, and Johnsen score in both treatment groups significantly increased compared to those of group 1 and group 2 (p < 0.05). The number of germinal epithelial cells in the 45 g/kg/day dose group increased compared to the induced dysfunction group and the distilled water group, particularly for spermatids (p < 0.05)

Conclusion: The herbal formula Hoa dam sinh tinh decoction increases seminiferous tubule diameter, germinal epithelium height, and germ cell count, and improves the Johnsen score compared to the induced dysfunction group when administered at a dose of 45 g/kg/day in an experimental model.

References
[1]
Tân Bân, Trịnh Trạch Đường. Nam khoa Trung Tây y kết hợp. Nhà xuất bản Giáo dục Cao đẳng Quảng Đông, Quảng Đông, Trung Quốc, 2012. Google Scholar
[2]
Bộ Y tế. Dược điển Việt Nam V. Nhà xuất bản Y học, Hà Nội. 2017. Google Scholar
[3]
Bộ Y tế. Hướng dẫn quy trình kỹ thuật chuyên ngành y học cổ truyền, Hà Nội, 2020. Google Scholar
[4]
Bộ Y tế. Hướng dẫn thử nghiệm tiền lâm sàng và lâm sàng thuốc đông y, thuốc từ dược liệu (Ban hành kèm theo Quyết định số 141/QĐ-K2ĐT ngày 27/11/2015), Hà Nội, 2015. Google Scholar
[5]
Johnsen S.G. Testicular biopsy score count - a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormones, 1970, 1 (1): 2-25. doi: 10.1159/000178170. Google Scholar
[6]
Mehranjani M.S, Noorafshan A, Momeni H.R et al. Stereological study of the effects of vitamin E on testis structure in rats treated with para-nonylphenol. Asian J Androl, 2009, 11(4): 508-516. doi: 10.1038/aja.2009.29. Google Scholar
[7]
Araujo Da Silva M.H, Buys-Gonçalves G.F et al. What is the effect of Tribulus terrestris on testicular morphology and sperm production? An experimental study in the rat model. Asian Journal of Andrology, 2024, 26 (1): 67-70. doi: 10.4103/aja202340. Google Scholar
[8]
Monteiro J.C, Pinto da Matta S.L, Predes F.S et al. Testicular morphology of adult Wistar rats treated with Rudgea viburnoides (Cham.) Benth. leaf infusion. Brazilian Archives of Biology and Technology, 2012, 55 (1): 101-105. doi: 10.1590/S1516-89132012000100013. Google Scholar
[9]
Koohkan F, Azarnia M, Bardeei L.K. The antioxidant and therapeutic effects Google Scholar
[10]
of Malva sylvestris extract on testicular tissue and sperm quality in varicocele-induced adult Wistar rats. Basic and Clinical Andrology, 2025, 35 (1): 22. doi: 10.1186/s12610-025-00271-4 Google Scholar
[11]
Hamidi H, Tofighi A, Azar J.T et al. Effect of Crocin and treadmill exercise on spermatogenesis and testis structure in Streptozotocin-induced diabetic rats: an experimental study. Crescent Journal of Medical and Biological Sciences, 2023, 10 (2): 98-104. doi: 10.34172/cjmb.2023.15. Google Scholar