Articles Vol. 67 No. CĐ8-NCKH 06/07/2026

INVESTIGATION OF THE ANTIMICROBIAL ACTIVITY AND CANDIDA ALBICANS KILL-KINETICS OF 0.12% CHLORHEXIDINE DIGLUCONATE MOUTHWASH IN DENTISTRY

Ly Công Thanh1,2, Nguyen Ngoc Anh1,2, Nguyen Khac Tiep1,3, Nguyen Duc Thien1,3
1 Hanoi University of Pharmacy
2 Trường Đại học Dược Hà Nộ
3 Trường Đại học Dược Hà Nội
DOI: 10.52163/yhc.v67iCD8.5690
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Keywords
Chlorhexidine digluconate, antimicrobial, C.albicans, mouthwash, MIC/MBC.
Abstract

Objective: The study aimed to evaluate the broad-spectrum antimicrobial activity and killing kinetics of a mouthwash containing 0.12% Chlorhexidine Digluconate (CHX-D) combined with 3% PEG4000.

Research methods: Antimicrobial activity was determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against 4 strains: E.coli, S.aureus, S.mutans, and C.albicans. Concentration-dependent effects (1-16 × MIC) were assessed for all strains, while time-kill kinetics (10-60 seconds) and microbial density (10⁵-10⁶ CFU/ml) were evaluated for C.albicans.

Results: Mouthwash CHX-D 0.12% showed strong activity with MBC/MIC ≤ 2 for bacteria and ≤ 4 for C.albicans. The solution eliminated > 99.9% of C.albicans after 10 seconds at 10⁵ CFU/ml and reached 99.99% after 40 seconds; at 10⁶ CFU/ml, it still achieved 99% after 10 seconds and complete killing after 60 seconds.

Kết luận: Kết quả khẳng định nước súc miệng CHX-D 0,12% là chế phẩm kháng khuẩn phổ rộng, diệt vi sinh vật nhanh và hiệu quả.

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