Articles Vol. 64 No. 1 27/12/2022

3. ANTI-CANCER EFFECT OF NATURAL KILLER CELLS IN AN IMMUNODEFICIENT MOUSE MODEL BEARING HUMAN LUNG CANCER TUMOR

Nguyen Thi Thuy Mau1,2, Tran Van Khanh1,2, Nguyen Linh Toan3,4, Tran Van Chieu1,2, Nguyen Thanh Binh1,2, Tran Huy Thinh1,2
1 Hanoi Medical University
2 Trường Đại học Y Hà Nội
3 Vietnam Military Medical University
4 Học viện Quân Y
Corresponding author: tranhuythinh@hmu.edu.vn
DOI: 10.52163/yhc.v64i1.565
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Keywords
Tế bào diệt tự nhiên, ung thư phổi, chuột thiếu hụt miễn dịch.
Abstract

Objectives: To evaluate the anti-cancer effect of natural killer cells in an immunodeficient mouse
model bearing human lung cancer tumor (H460 lineage).
Objects and methods: Natural killer (NK) cells were activated, differentiated, and proliferated in the
laboratory until a concentration of at least 107 cells/ml was reached. Immune-deficient mice (nude
mice) 6 - 8 weeks old, number of 24 animals, were injected with 106 H460 lineage human lung cancer
cells under the skin of the thigh to create human lung cancer in nude mice. When the tumor size was
about 50 - 70mm3 (after 10 days of transplantation), mice were divided into 4 groups (6 animals/
group), the treatment groups were injected with NK cells through the tail vein with a dose of 106
cells per 10g, 5x106 cells per 10g and 107 cells per 10g, respectively, once a week, for 3 consecutive
weeks, the control group was injected with 0,9% NaCl containing 5% human albumin.
Results: The group of mice with H460 human lung cancer treated with NK cells had a smaller tumor
volume, a longer survival time and a lower death rate than the control group with p>0,05 due to small
sample size.
Conclusion: The study suggests that natural killer cells were effective against human lung cancer in
an immune-deficient mouse model of xenograft cancer.

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