Articles Vol. 67 No. CĐ4-HN Ký sinh trùng-HN Hóa sinh 2026 21/04/2026

CARBAPENEMASE GENE SPECTRUM AND COLISTIN SUSCEPTIBILITY OF PSEUDOMONAS AERUGINOSA ISOLATES FROM INPATIENTS AT NGUYEN TRI PHUONG HOSPITAL

Nguyen Minh Ha1,2,3,4, Nguyen Quang Huy1,3
1 Center for Biomedical Testing and Research, Pham Ngoc Thach University of Medicine
2 Laboratory Department, Nguyen Tri Phuong Hospital
3 Trung tâm Xét nghiệm và Nghiên cứu Y sinh, Trường Đại học Y khoa Phạm Ngọc Thạch
4 Khoa Xét nghiệm, Bệnh viện Nguyễn Tri Phương
Corresponding author: nguyenminhha@pnt.edu.vn
DOI: 10.52163/yhc.v67iCD4.4846
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Keywords
Pseudomonas aeruginosa, blaNDM carbapenemase genes, Carbapenem resistance, Colistin resistance.
Abstract

of Pseudomonas aeruginosa isolates collected from inpatients at Nguyen Tri Phuong Hospital during 2024-2025.

Methods: 88 non-duplicate isolates underwent antimicrobial susceptibility testing (CLSI 2025), Colistin MIC determination by E-test, and multiplex real‑time PCR screening for blaNDM, blaIMP, blaVIM, blaKPC and mcr-1-mcr-10 genes.

Results: Resistance to Imipenem and Meropenem ranged from 42-46%, whereas all isolates remained susceptible to Colistin. Among 32 carbapenemase‑producing isolates, blaNDM predominated (over 50%), surpassing blaIMP (28-29%), with a strong correlation between blaNDM/blaIMP carriage and Carbapenem-resistant phenotypes (p < 0.00001). No mcr variants were detected.

Conclusion: The predominance of blaNDM within the P.aeruginosa carbapenemase spectrum, alongside preserved Colistin susceptibility, underscores the importance of molecular surveillance to optimize therapy and strengthen antimicrobial stewardship in hospital settings.

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