Articles Vol. 66 No. CĐ11-NCKH 22/08/2025

52. SEDATIVE AND ANALGESIC ACTIVITY OF NEPETA CATARIA L.

Ha Quang Thanh1,2, Tran Thi Viet Nga3,4, Huynh Chi Thien1,2, Au Duong Tuyet Mai1,2, Dinh Thi Huong Giang1,2, Tran Huynh Thao Nguyen1,2, Bui The Vinh5,6, Nguyen Thi Le Thuy2,5
1 Center for Biotechnology
2 Trung Tâm Công Nghệ Sinh Học
3 Academy of Science and Technology
4 Học Viện Khoa Học và Công Nghệ
5 Hong Bang International University
6 Trường Đại Học Quốc Tế Hồng Bàng
Corresponding author: quangthanh.sly@gmail.com
DOI: 10.52163/yhc.v66iCD11.3035
30 Views
16 Downloads
Keywords
Sedation anesthesia hot–plate test light–dark box test Nepeta cataria
Abstract

Objective: To investigate the sedative and analgesic effects of aqueous and 99% ethanol extracts from Nepeta cataria L.

Materials  and  methods: The sedative-anxiolytic effects were evaluated using the pentobarbital-induced sleeping test and the light-dark box test. The analgesic effects were assessed through the acetic acid-induced writhing test in C57BL/6 mice.

Results: The Nepeta cataria ethanol extract at a dose of 162 mg/kg demonstrated sedative-anxiolytic effects by prolonging pentobarbital-induced sleeping time and increasing the time spent in the light area, with effects comparable to Diazepam (1 mg/kg). Additionally, the 162 mg/kg ethanol extract exhibited analgesic effects by reducing the number of writhings induced by acetic acid which was similar to the effect of diclofenac potassium (15 mg/kg).

Conclusion: Oral administration of the Nepeta cataria ethanol extract at 162 mg/kg demonstrated sedative and analgesic effects in mouse models. The aqueous extract at 70 mg/kg did not exhibit significant effects in the conducted experiments.

References
[1]
Gallup. Gallup Global Emotions Report 2019. Gallup, 2019. Google Scholar
[2]
Finan R, Goodin T. The association of sleep and pain: an update and a path forward. The Journal of Pain, 2013, 14(12), 1539-52. Google Scholar
[3]
Ali B , Al-Wabel N A, Khan S. A, Anwar F. Essential oils used in aromatherapy: A systemic review. Asian Pacific Journal of Tropical Biomedicine, 2021 11(5), 201–214 Google Scholar
[4]
Bộ Y Tế. Hướng dẫn thử nghiệm tiền lâm sàng và lâm sàng thuốc đông y, thuốc từ dược liệu. Quyết định số 141/QĐ-K2ĐT, 2015. Google Scholar
[5]
Lê Hoàng Anh Phú, Nguyễn Thị Thu Hương. Khảo sát tác dụng chống stress của cao chiết từ lá và đọt non lạc tiên tây di thực trồng tại Việt Nam trên mô hình chuột bị stress cô lập. Tạp chí Dược liệu, 2015 20(6), 368-373. Google Scholar
[6]
Nguyễn Thị Thu Hương, Đào Trần Mộng. Tác dụng trên hệ thần kinh trung ương của các cao chiết từ lá và đọt non lạc tiên tây di thực trồng tại Việt Nam. Tạp chí Dược liệu, 2015, 20(2), 116-121. Google Scholar
[7]
Bourin M, Hascoët M. The mouse light/dark box test. European Journal of Pharmacology, 2003, 463(1-3), 55-65. Google Scholar
[8]
Whittle BA. The use of changes in capillary permeability in mice to distinguish between narcotic and non-narcotic analgesics. British Journal of Pharmacology and Chemotherapy, 1964, 22(2), 246-253.. Google Scholar
[9]
Makkar SR, Zhang SQ. Behavioral and neural analysis of GABA in the acquisition, consolidation, reconsolidation, and extinction of fear memory. Neuropsychopharmacology, 2010, 35(8), 1625-1652. Google Scholar
[10]
Popik P, Kostakis E. The Anxioselective Agent 7-(2-Chloropyridin-4-yl)pyrazolo-[1,5-a]-pyrimidin-3-yl](pyridin-2-yl) methanone (DOV 51892) Is More Efficacious Than Diazepam at Enhancing GABA-Gated Currents at α1 Subunit-Containing GABAAReceptors. Journal of Pharmacology and Experimental Therapeutics, 2007, 319(3), 1244-1252 Google Scholar
[11]
Zhang Q .Nepetalactone modulates GABAA receptor activity in rodent neurons. Biochemical Pharmacology, 2018 156, 214-222. Google Scholar
[12]
Aydin S., Beis R, Baser C. Nepeta cataria L. as a sedative and hypnotic agent: its interaction with pentobarbital and diazepam in mice. Phytotherapy Research, 1998, 12(1), 60–62. Google Scholar
[13]
Rezvani ME, Roohbakhsh A, Esmaeili H. Anticonvulsant and anxiolytic effects of Nepeta menthoides Boiss. & Buhse in mice. Journal of Ethnopharmacol. 2010, 129(2), 185-190. Google Scholar
[14]
Derardt R, Falhout M. Release of prostaglandins E and F in an algogenic reaction and its inhibition. European Journal of Pharmacology, 1980, 61(1), 17–24. Google Scholar
[15]
Reichert. Nepetalactone’s sedative effects involve opioid receptor modulation in mice. Psychopharmacology, 2020, 237(5), 1233–1244. Google Scholar
[16]
Aydin S, Beis R, Ozturk Y, Baser C. Nepetalactone: A new opioid analgesic from Nepeta caesarea Boiss. Phytother Res. 1998,12(2), 157-159. Google Scholar
[17]
Formisano C, Rigano D, Senatore F, Bruno M, Rosselli S, Bellone G. Chemical composition and biological activity of Nepeta cataria essential oil. Chem Biodivers. 2011, 8(7),1157-1171. Google Scholar