Articles Vol. 66 No. CĐ8-NCKH 22/08/2025

27. DEVELOPMENT AND VALIDATION OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE QUANTIFICATION OF AFLATOXIN B1 IN HERBAL DRUGS AND CEREALS

Chu Van Men1,2, Pham Huy Hoang1,2, Nguyen Van Chuyen1,2, Nguyen Van Ba1,2, Nguyen An Bao Chau3,4, Do Quang Minh5,6, Ngo Dinh Nhan7,8, Ngo Hoang Yen Nhi7,9
1 Vietnam Military Medical University
2 Học viện Quân y
3 Vinschool Smart City Secondary and High School
4 Trường Trung học cơ sở và Trung học phổ thông Vinschool Smart City
5 Le Loi High School
6 Trường Trung học phổ thông Lê Lợi
7 Drug Administration, Ministry of Health
8 Cục Quản lý Dược, Bộ Y tế
9 Cục Quản lý Dược, BộY tế
Corresponding author: chuvanmen@vmmu.edu.vn
DOI: 10.52163/yhc.v66iCD8.2573
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Keywords
Herbal drugs, cereals Aflatoxin B1 high-performance liquid chromatography.
Abstract

Objectives: A high-performance liquid chromatography method was developed and validated for the quantification of Aflatoxin B1 in Herbs and cereals.

Method: The proposed method was validated according to ICH guidelines for specificity, repeatability, linearity, accuracy, and precision.

Results: The method was achieved including Waters C18 column (50 mm × 2.1 mm; 1.7 µm), the mobile phase contained aqueous 0.1% phosphoric acid:acetonitrile 0.2% (33:67) in isocratic with flow rate of 0.2 ml/min, injection volume of 20 μl, and detector PDA-UV at 354 nm. The developed method showed the present of Aflatoxin B1 in 2 Herbs and cereals samples, account for about 2% of samples analyzed.

Conclusion: The study was successfully developed and validated high-performance liquid chromatography method for quantification of Aflatoxin B1 in Herbs and cereals. This is the background for further studies.

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