Articles Vol. 66 No. 3 26/05/2025

49. THE MOUTHWASH CONTAINING CHLORHEXIDINE AND CHLORINE DIOXIDE: AN IN VITRO STUDY

Tran Thi Phuong Thao1,2, Dang Thi Tham1,2, Huynh To Tram1,2, Tran Dieu Linh1,2, Tran Thanh Dat1,2
1 Faculty of Odonto-Stomatology, Hong Bang Interntaional University
2 Khoa Răng Hàm Mặt, Trường Đại học quốc tế Hồng Bàng
Corresponding author: thaottp@hiu.vn
DOI: 10.52163/yhc.v66i3.2539
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Keywords
chlorhexidine chlorine dioxide human gingival fibroblast mouthwash.
Abstract

Objective: To evaluate the effect of the mouthwash containing chlorhexidine and chlorine dioxide on human gingival fibroblasts.

Subject and method: The impact of mouthwash containing both chlorhexidine (concentrations from 0.01, 0.02, 0.05, 0.1, and 0.2%) and chlorine dioxide (0.05 and 0.1%) on human gingival fibroblasts after 24 hours of contact was assessed using the colorimetric assay MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], in accordance with ISO10993-5:2009.

Results: After 24 hours of contact, the mouthwash containing chlorhexidine and chlorine dioxide showed toxicity grades 3 and 4 to the human gingival fibroblast.

Conclusion: The mouthwash containing chlorhexidine and chlorine dioxide was toxic to human gingival fibroblasts after 24 hours of exposure.

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