Articles Vol. 66 No. CĐ4-NCKH 15/04/2025

31. STUDY ON Pvcrt GENE MUTATIONS ASSOCIATED WITH CHLOROQUINE RESISTANCE IN PLASMODIUM VIVAX IN MUONG TE, LAI CHAU USING real-time PCR AND WHOLE-GENOME SEQUENCING

Nguyen Quang Thieu1,2, Nguyen Thi Hong Ngoc1,2, Nguyen Thi Phuong3,4, Nguyen Thi Huong Binh1,2, Nguyen Van Hong1,2, Nguyen Duc Giang1,2
1 National Institute of Malariology, Parasitology and Entomology
2 Viện Sốt rét, Ký sinh trùng và Côn trùng Trung ương
3 Hanoi University of Public Health
4 Trường Đại học Y tế Công cộng
Corresponding author: thieunq@gmail.com
DOI: 10.52163/yhc.v66iCD4.2349
14 Views
15 Downloads
Keywords
Plasmodium vivax Pvcrt Chloroquine resistance mutation gene expression Vietnam
Abstract

Objective: This study investigates Pvcrt gene expression and mutations associated with Chloroquine resistance in Plasmodium vivax isolates from Muong Te district, Lai Chau province, Vietnam.

Methods: A cross-sectional study was conducted on 48 blood samples positive for P. vivax, collected from October 2022 to March 2024. Pvcrt expression was quantified by qPCR, and mutations were identified using whole genom Illumina sequencing. In addition, a literature review (2008-2023) was performed to evaluate Pvcrt roles globally.

Results: 14.6% of samples showed increased Pvcrt expression. C350T mutation was present in 4.2% and codon 10 insertions (K10) in 12.5%. C350T was significantly associated with overexpression (p < 0.05). Literature confirms Pvcrt involvement in Chloroquine resistance across Southeast and South Asia.

Conclusion: Pvcrt plays a potential role in Chloroquine resistance surveillance in P. vivax. Integration of molecular monitoring of Pvcrt into Vietnam’s malaria control strategies is recommended.

References
[1]
World Health Organization, World Malaria Report 2021, Geneva, 2021. Google Scholar
[2]
Baird J.K, Hoffman S.L, White N.J, Resistance to therapies for infection by Plasmodium vivax, Clin Microbiol Rev. 2009, 22 (3): 508-534. Google Scholar
[3]
Melo G.C, Monteiro W.M, Siqueira A.M et al, Expression levels of pvcrt-o and pvmdr-1 are associated with chloroquine resistance and severe Plasmodium vivax malaria in patients of the Brazilian Amazon, PloS One, 2014, 9 (8): e105922. Google Scholar
[4]
Barnadas C, Ratsimbasoa A, Tichit M et al, P. vivax resistance to Chloroquine in Madagascar: clinical efficacy and polymorphisms in pvcrt-o and pvmdr1 genes, Antimicrob Agents Chemother, 2008, 52 (12): 4233-4240. Google Scholar
[5]
Sharma A, Rawal C, Jindal A et al, Expression pattern of pvcrt-o and polymorphisms in P. vivax isolates from India, Acta Tropica, 2022, 230: 106370. Google Scholar
[6]
Amato R, Pearson R.D, Almagro-Garcia J et al, Origins of the current outbreak of multidrug-resistant malaria in Southeast Asia, Nat Microbiol, 2018, 3: 1195-1205. Google Scholar
[7]
Cingolani P, Platts A, Wang L.L et al, A program for annotating and predicting the effects of SNPs, SnpEff, Fly, 2012, 6 (2): 80-92. Google Scholar
[8]
Miotto O, Amato R, Ashley E.A et al, Genetic architecture of artemisinin-resistant Plasmodium falciparum, Nat Genet, 2015, 47 (3): 226-234. Google Scholar
[9]
McKenna A, Hanna M, Banks E et al, The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data, Genome Res, 2010, 20 (9): 1297-1303. Google Scholar
[10]
Li H, Durbin R, Lam T.W et al, Fast and accurate short read alignment with Burrows-Wheeler transform, Bioinformatics, 2009, 25 (14): 1754-1760. Google Scholar
[11]
Alexander D.H, Novembre J, Lange K, Fast model-based estimation of ancestry in unrelated individuals, Genome Res, 2009, 19 (9): 1655-1664. Google Scholar
[12]
Price R.N, Douglas N.M, Anstey N.M, New developments in Plasmodium vivax malaria: severe disease and the rise of Chloroquine resistance, Curr Opin Infect Dis, 2009, 22 (5): 430-435. Google Scholar