Articles Vol. 66 No. CĐ6-HNKH Bệnh viện Sản nhi Nghệ An 31/05/2025

35. THE RESULTS OF BLASTOCYST FORMATION RATE WHEN INJECTING HIGH DNA FRAGMENTATION INDEX SPERM INTRA-CYTOPLASMIC

Le Ngoc Tue Nhi1,2, Pham Thai Ha1,2, Dao Xuan Kien1,2, Nguyen Dieu Linh1,2
1 Phu Tho Obstetric and Pediatric Hospital
2 Bệnh viện Sản Nhi Phú Thọ
Corresponding author: lengoctuenhi1001@gmail.com
DOI: 10.52163/yhc.v66iCD6.2300
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Keywords
High sperm DNA fragmentation index ICSI blastocyst
Abstract

Objective: To evaluate the results of blastocyst formation rate injecting sperm with high fragmentation index (DFI ≥ 30) incubated with follicular fluid and injected intra-cytoplasmic. Subjects and Methods: A cross-sectional descriptive study was conducted on 1,383 oocytes from 110 couples undergoing in vitro fertilization (IVF) at the Center for Assisted Reproduction and Andrology - Phu Tho Obstetrics & Pediatric Hospital. The fertilization results, embryo formation (Day 3) and blastocyst formation (Day 5 and 6) were compared between the study group (injected high DFI sperm incubated with follicular fluid) and the control group (injected high DFI sperm not incubated with follicular fluid).

Results: The fertilization rate, Day 3 embryo rate, Day 3 embryo rate (grade 1 + grade 2), blastocyst rate and blastocyst rate (grade 1+ grade 2) of the study group compared to the control group showed statistically significant differences: 90.4% vs. 81.6%, p < 0.05; 100% vs. 99.2%, p < 0.05; 57.5% vs. 50.9%, p < 0.05;  65.7% vs. 47.7%, p < 0.05; and 63.5% vs. 32.4%, p < 0.05, respectively.

Conclusion: The fertilization rate and the blastocyst formation rate in the study group higher the control group, particularly the blastocyst rate (grade 1+ grade 2) in the sudy group with double for the control group.

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