Articles Vol. 62 No. 7 (2021) 09/12/2021

USE MULTIPLEX REALTIME PCR METHODS FOR RAPID DETECTION OF FIVE BACTERIAL PATHOGENS CAUSING NOSOCOMIAL INFECTION: STAPHYLOCCCUS AUREUS, ESCHERICHIA COLI, PSEUDOMONAS AERUGINOSA, KLEBSIELLA PNEUMONIAE, ACINETOBACTER BAUMANNII ISOLATED FROM RESPIRATORY SAMPL

Nguyen Thi Loan1,2, Nguyen Trong Linh3,4, Nguyen Minh Hien5,6, La Thi Huyen3,4
1 Graduate University of Science and Technology, Vietnam Academy of Science and Technology
2 Học Viện Khoa học và Công nghệ, Viện Hàn lâm KH&CN Việt Nam
3 Insitute of Biotechnology, Vietnam Academy of Science and Technology
4 Viện Công nghệ Sinh học, Viện Hàn Lâm KH&CN Việt Nam
5 Thanh Nhan Hospital
6 Bệnh viện Thanh Nhàn
Corresponding author: ntloan@hluv.edu.vn
DOI: 10.52163/yhc.v62i7 (2021).214
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Keywords
Respiratory specimens, Multiplex real-time PCR, nosocomial infection, nosocomial pathogens.
Abstract

Purposes: Using the multiplex realtime PCR assay to simultaneously detect five nosocomial pathogens: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetorbacter bauminnii isolated from respiratory specimens. Objectives and Methods: 47 respiratory specimens from patients were treated at Thanh Nhan and Duc Giang hospitals as nosocomial infection. The patient samples were divided into 2 parts, one part was used for culture test and the other part was performed by multiplex real-time PCR assay. Results: 40/47 samples were positive (85.1%), the multiplex real-time PCR method can simultaneously detect 5 nosocomial pathogens from respiratory specimens, similarity coefficient with the culture method is 0.97 (p<0.05).

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