Articles Vol. 66 No. CĐ3-HNKH Ký Sinh Trùng VN lần thứ 51 24/03/2025

17. REARING OF THE HOUSE DUST MITE DERMATOPHAGOIDES FARINAE IN THE LABORATORY

Dao Minh Trang1,2, Hoang Dinh Canh1,2, Bui Le Duy1,2, Nguyen Van Dạt1,2, Phung Thi Phuong Thao1,2, Nguyen Van Dung1,2
1 National Institute of Malariology, Parasitology, and Entomology
2 Viện Sốt rét - Ký sinh trùng - Côn trùng - Trung ương
Corresponding author: daominhtrangnimpe@gmail.com
DOI: 10.52163/yhc.v66iCĐ3.2137
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Keywords
Dermatophagoides farinae rearing
Abstract

Objective: To determine the optimal conditions for rearing house dust mites Dermatophagoides farinae in the laboratory.

Methods: The study was conducted from July to December 2024 at the National Institute of Malariology, Parasitology, and Entomology. Evaluating the effects of different mite rearing densities on the growth and development of the house dust mite Dermatophagoides farinae. Evaluating the effects of different mite rearing food environments on the growth and development of the house dust mite Dermatophagoides farinae.

Results: In the 5 kind of rearing culture environments, the rearing culture environment 5th (includes dried shrimp powder, bread flour, cereal powder in the ratio of 4:7:1 and supplemented with Blomia tropicalis mites in the ratio of 2Dermatophagoides farinae:1Blomia tropicalis) gave the best results for house dust mite growth. The number of house dust mites increased 198.37 times after 8 weeks and 585.5 times after 12. Rearing culture environment 1st (including dried shrimp powder, bread flour, cereal powder in the ratio of 4:7:1) and environment 2nd (including dried shrimp, bread flour, cereal powder, yeast in the ratio of 4:7:1:2) gave the results for house dust mite growth from 93.47-96.23 times after 8 weeks and 237.03-259.35 times after 12 weeks.. The best growth density of house dust mites was at 50 mites/plate with the number of mites increasing 198.37 times and 200.83 times after 8 weeks in the the kind 10g rearing culture environments/plate and 585.50 times and 529.52 times after 12 weeks in the 20g rearing culture environment/ plate.

Conclusion: The optimal conditions for house dust mites Dermatophagoides farinae at temperature of 25 - 280C, humidity: 75 - 80%, light: dark time is 14:10 hours with the rearing culture environment including dried shrimp powder, bread flour, cereal powder in the ratio: 4:7:1 and supplemented with Blomia tropicalis mites in the ratio of 2:1 at a house dust mite culture density of 50 mites/10g rearing culture environment/plate.

References
[1]
. Fain A., Guerin B, Hart B.J, (1990), Mite and Allergic disease. Allerbio. F-55270 varennes argonne, France: 190pp Google Scholar
[2]
. Phạm Quang Chinh, Vũ Minh Thục, Đái Duy Ban (2002), “Kết quả điều tra 2 loài mạt bụi nhà gây dị ứng Glycyphagus domestic và Dermatophagoides pteronyssinus ở hà Nội và các vùng lân cận”, Báo cáo hội nghị Côn trùng toàn quốc (lần thứ 4), NXB Nông nghiệp, tr 94-99. Google Scholar
[3]
. Miyamoto, J., A. Ishii and M. Sasa (1975), “A successful method for mass culture of the house dust mute, Dermatophagoides pteronyssinus (Trousart, 1897)”. Jpn. J. Exp. Med., 45: 133-138. Google Scholar
[4]
. Vũ Minh Thục (1997), Một số đặc điểm dị nguyên mạt bụi nhà, Tạp chí Y học thực hành, N4, (19 – 21). Google Scholar
[5]
. Colloff, M.J., (1985), The biology and control of the European house dust mite Dermatophagoides pteronyssinus (Trouessart, 1897) (Acari: Pyroglyphidae) in relation to atopic allergy. Ph.D. thesis, University of Glasgow, 104 pp. (unpublished). Google Scholar
[6]
. Huỳnh Quang Thuận, Vũ Minh Thục, Võ Thanh Quang, Đinh Văn Minh (2009), “Nuôi cấy mạt bụi nhà Dermatophagoides pteronyssninus” Y học thực hành, Số 8, (39 – 41). Google Scholar