Articles Vol. 66 No. CĐ3-HNKH Ký Sinh Trùng VN lần thứ 51 24/03/2025

10. COMPARISON OF DIAGNOSTIC VALUE BETWEEN CHEMICAL TESTS FOR LARGE LIVER FLUKE INFECTION AT THE QUY NHON INSTITUTE OF MALARIA - PASTETRY - ENTO IN 2024

Dao Trinh Khanh Ly1,2, Nguyen Duc Chinh1,2, Nguyen Xuan Thien1,2, Huynh Hong Quang1,2, Ho Van Hoang1,2
1 INSTITUTE OF MALARIOLOGY, PARASITOLOGY AND ENTOMOLOGY, QUY NHON
2 Viện Sốt rét - Ký sinh trùng - Côn trùng Quy Nhơn
Corresponding author: khanhly75@yahoo.com
DOI: 10.52163/yhc.v66iCĐ3.2127
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Keywords
Fascioliasis ELISA
Abstract

Fasciola spp. in humans is currently a common disease in Vietnam, especially in the Central Highlands. The disease has symptoms similar to many other digestive and hepatobiliary diseases, so it is easy to misdiagnose and treat late. The gold standard for diagnosing the disease is finding fluke eggs in the stool, but this is rarely observed. In practice, the diagnosis of the disease is mainly based on clinical symptoms, liver damage on imaging and testing to detect antibodies against Fasiola spp. in the serum. This study was conducted to compare the similarity or diagnostic value between commercial ELISA diagnostic test reagents currently circulating in Vietnam. Each reagent set was tested on 34 serum samples of healthy people and 34 serum samples of cases infected with Fasciola spp. determined by clinical symptoms, liver lesions on ultrasound, Western blot test for antibodies against Fasciola spp.. The results showed that the sensitivity of ELISA reagents of Viet Sinh company produced in Vietnam, Cortez produced in the US, DRG produced in Germany, NewLife produced in the US were 91.18%, 91.18%, 100%, 91.18%, respectively; The specificity was 97.06%, 85.29%, 97.06%, 94.18%, and the accuracy was 94.12%, 88.23%, 98.53%, 92.65%, respectively. Compared with the confirmed cases, the Kappa coefficient of the 4 test chemicals was 0.882; 0.765; 0.971; 0.853, respectively. Comparing the agreement between the 4 test chemicals, DRG, Viet Sinh, and New Life had the highest similarity coefficient (Kappa > 0.8). Therefore, the 4 commercial chemicals tested in the study all had good diagnostic value.

References
[1]
Mas-Coma S, Valero MA, Bargues MD. Human and Animal Fascioliasis: Origins and Worldwide Evolving Scenario. Clin Microbiol Rev. Dec 21 2022;35(4):e0008819. doi:10.1128/cmr.00088-19 Google Scholar
[2]
Tran Vinh Hien, Tran Thi Kim Dung, Nguyen Huu Chi, Phan Huu Danh, Pham Thi Hanh. Fascioliasis in Vietnam. Proceedings of the 3rd seminar on food-borne parasitic zoonoses: Food- and water- borne parasitic zoonoses in the 21st century. Southeast Asian Journal of Tropical Medicine and Public Health. Volume 32- Supplement 2, 2001. Google Scholar
[3]
Đào Trịnh Khánh Ly. Tình hình bệnh nhân nhiễm giun sán được khám phát hiện tại Phòng khám Viện Sốt rét - Ký sinh trùng - Côn trùng Quy Nhơn. Tạp chí Phòng chống bệnh sốt rét và các bệnh ký sinh trùng. 2017;96:105-111. Google Scholar
[4]
Sarkari B, Khabisi SA. Immunodiagnosis of Human Fascioliasis: An Update of Concepts and Performances of the Serological Assays. J Clin Diagn Res. 2017;11(6):OE05-OE10. doi:10.7860/JCDR/2017/26066.10086 Google Scholar
[5]
Jonathan J. Deeks, Patrick M. Bossuyt, Mariska M. Leeflang, Yemisi Takwoingi. Cochrane Handbook for Systematic Reviews of Diagnostic Test Accuracy. The Cochrane Collaboration and John Wiley & Sons Ltd, 2023, UK, page 46. Google Scholar
[6]
Na T. D. Tran, Phuong Anh Ton Nu, Kitti Intuyod, Ly T. K. Dao, Porntip Pinlaor, Yukifumi Nawa, Kiattawee Choowongkomon, Amornrat Geadkaew-Krenc, Nanthawat Kosa, Rudi Grams, and Somchai Pinlaor (2019). Evaluation of a commercial enzyme-linked immunosorbent assay kit and in-house Fasciola gigantica cysteine proteinases-based enzyme-linked immunosorbent assays for diagnosis of human fascioliasis. Am. J. Trop. Med. Hyg. 100, 591–598. https://doi.org/10.4269/ajtmh.18-0833 Google Scholar
[7]
Roxana del Carmen Medina-Rojas, Higinio Alberto Zuñiga-Sanchez, Isabel Eveling Castillo-Coaquira, Wilson Gregorio Sucari-Turpo, Zeida Patricia Hoces-La-Rosa, Ymelda Sarayasi-Alencastre. Wester Blot for the diagnosis of the acute and chronic phase of animal and human fasciolosis, using different antigens of Fasciola hepatica. Journal of Survey in Fisheries Sciences. 2023;10(3S):1362-1373. Google Scholar
[8]
Intapan PM, Maleewong W, Wongkham C, Tomanakarn K, Ieamviteevanich K, Pipitgool V, et al. Excretory-secretory antigenic components of adult Fasciola gigantica recognized by infected human sera. Southeast Asian J Trop Med Public Health. 1998;29(3):579-83 Google Scholar
[9]
Nguyen TG, Le TH, De NV, et al. Assessment of a 27-kDa antigen in enzyme-linked immunosorbent assay for the diagnosis of fasciolosis in Vietnamese patients. Trop Med Int Health. 2010;15(4):462-467. doi:10.1111/j.1365-3156.2010.02468.x Google Scholar
[10]
Shafiei R, Sarkari B, Sadjjadi SM. Performance of a 27 kDa Fasciola hepatica Antigen in the Diagnosis of Human Fascioliasis. J Lab Physicians. 2015 Jan-Jun;7(1):17-20. doi: 10.4103/0974-2727.154781. PMID: 25949054; PMCID: PMC4411804. Google Scholar
[11]
Attallah AM, Bughdadi FA, El-Shazly AM, Ismail H. Immunodetection of Fasciola gigantica circulating antigen in sera of infected individuals for laboratory diagnosis of human fascioliasis. Clin Vaccine Immunol. 2013;20(10):1569-1577. doi:10.1128/CVI.00305-1320. Google Scholar