Articles Vol. 66 No. CĐ1-NCKH 24/01/2025

13. GENOTYPE CHARACTERISTICS OF B. PSEUDOMALLEI STRAINS ISOLATED FROM CHILDREN DIAGNOSED WITH WHITMORE AT THE NATIONAL CHILDREN’S HOSPITAL

Le Thi Yen1,2, Nguyen Van Lam1,2, Tran Thanh Duong3,4, Phan Thi Thu Chung1,2, Hoang Thi Bich Ngoc2,5, Tran Minh Dien2,5
1 Vietnam National Children’s Hospital
2 Bệnh viện Nhi Trung ương
3 National Institute of Nutrition
4 Viện Dinh dưỡng Quốc gia
5 Vietnam National Children's Hospital
Corresponding author: Bslethiyen2111@gmail.com
DOI: 10.52163/yhc.v66iCD1.1975
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Keywords
Whitmore disease B. pseudomallei strains sequence type children
Abstract

Background: Burkholderia pseudomallei is the main cause of Whitmore disease in humans and animals. It is mainly prevalent in tropical Southeast Asia and Northern Australia with a high mortality rate, the disease is likely to recur and cause persistent infection.

Objective: To determine the genetic characteristics of B. pseudomallei strains isolated from pediatric patients with Whitmore disease treated at the National Children’s Hospital.

Methods: The study was conducted at the National Children’s Hospital from 2017 to 2023. A total of 37 strains of B. pseudomallei were isolated and cultured from clinical samples of pediatric patients diagnosed with Whitmore disease. Illumina sequencing technology successfully sequenced the entire genome of 37 bacterial strains.

Results: Among 37 B. pseudomallei strains, we detected 12 genotypes: ST67 was 10/37 strains (27%); ST221 and ST541 were 5/37 strains (13.5%); ST545 was 3/37 strains (8.1%); ST46, ST50, ST68, ST500, ST507, ST544 each had 1 strain. In addition, we also detected 1 strain with a new gene sequence belonging to the ST group with no information in the PubMLST database.

Conclusions: Subgroup ST67 is the common genotype of B. pseudomallei isolated from children diagnosed with Whitmore in the Northern provinces of Vietnam. This is the first study in Vietnam to publish a large number of whole genome sequences of B. pseudomallei strains.

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