Articles Vol. 66 No. CĐ1-NCKH 24/01/2025

7. COMPARISON OF RAPID CENTRIFUGATION WITH ROUTINE CENTRIFUGATION FOR SOME COAGULATION TESTING (PT, APTT, FIBRINOGEN) AT DEPARTMENT OF HEMATOLOGY IN VIETDUC UNIVERSITY HOSPITAL

Nguyen Thu Hanh1,2, Ha Van Phu1,2, Tran Thi Hang1,2, Doan An Son1,2, Nguyen Van Chinh1,2, Nguyen Manh Cuong1,2, Nguyen My Van1,2
1 Vietduc University Hospital
2 Bệnh viện Hữu nghị Việt Đức
Corresponding author: hanhhhtm@yahoo.com
DOI: 10.52163/yhc.v66iCD1.1968
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Keywords
Method comparison CLSI-EP09c rapid centrifugation
Abstract

Objectives: Comparison of rapid centrifugation (2300g in 5 minutes) with routine centrifugation (1500g  in 15 minutes) for some coagulation testing (PT, APTT and fibrinogen) at Department of Hematology in Vietduc University Hospital.

Methods: Experimental study on 45 pairs of patient samples according to the Clinical and Laboratory Standards Institute (CLSI) guideline for Method Comparison and Bias Estimation Using Patient Samples (EP09c).

Results: Platelet count after rapid centrifugation was 3.53 ± 1.50 G/l, similar to routine centrifugation (3.45 ± 1.58 G/l) with no statistically significant difference (p > 0.05). The results of Passing-Bablok linear regression analysis and Bland-Altman difference plot estimation showed that the difference in PT-INR and APTT-r and fibrinigen between the two centrifugation methods was within acceptable limits.

Conclusions: The present study showed no no statistically significant difference when comparable results between rapid (2300g × 5 minutes) and routine (1500g × 15 minutes) centrifugation techniques for some coagulation screening test (PT and APTT and fibrinogen). Rapid centrifugation can be used in emergency coagulation screening tests, especially in red code situations.

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