Articles Vol. 66 No. 1 07/01/2025

7. MOLECULAR IDENTIFICATION AND PHYLOGENETIC RELATIONSHIP OF DEMODEX MITES IN THE DEMODICOSIS’ PATIENTS BASED ON MITOCHONDRIAL 16S rDNA TECHNIQUE

Nguyen Ngoc Vinh1,2, Do Trung Dung3,4, Huynh Hong Quang1,2, Nguyen Thi Minh Trinh1,2, Nguyen Thi Thanh Quyen1,2, Nguyen Thi Lien Hanh1,2, Nguyen Duc Chinh1,2
1 Institute of Malariology, Parasitology, Entomology Quy Nhon
2 Viện Sốt rét - Ký sinh trùng - Côn trùng Quy Nhơn
3 National Institute of Malariology, Parasitology, and Entomology
4 Viện Sốt rét - Ký sinh trùng - Côn trùng Trung ương
Corresponding author: vinh.tmed.vn@gmail.com
DOI: 10.52163/yhc.v66i1.1904
15 Views
17 Downloads
Keywords
Demodex spp Demodex folliculorum mt 16S rDNA phylogenetic relationship
Abstract

Objectives: To determine of Demodex species and phylogenetic relationship by molecular techniques.

Methods: DNA were isolated from the demodicois patients’ Demodex mites and used for PCR amplification of mitochondrial (mt) 16S rDNA. The amplified PCR product were purified and used for molecular identification. The amplified mt16s rDNA product was sequenced and characterized to BLAST search in the NCBI database for molecular identification.

Results: All of 12 identified Demodex samples belongs to Demodex folliculorum. The phylogenetic tree constructed by using mt16s rDNA sequence suggests that D. folliculorum is closer to D. brevis. All the isolates belong to D. folliculorum and the mitochondrial DNA 16S rDNA partial sequence is applicable for phylogenetic relationship analysis, but not for interspecies identification.

Conclusions: All of 12 isolates belong to D. folliculorum, and the mitochondrial 16S rDNA partial sequence is applicable for phylogenetic relationship analysis at the genus-species level.

References
[1]
Fen Wei, Kevin J, Varghese, Hongxiang Chen (2024). Evidence for the clinical association between Demodexand rosacea: A review. Dermatology;240(1):95-102. Google Scholar
[2]
Gilbert Smith, Alejandro Manzano-Marín, Henk R. Braig (2022). Human follicular mites: Ectoparasites becoming symbionts. Mol. Biol. Evol. 39(6):msac125 Google Scholar
[3]
Hsu CK, Hsu MM, Lee JY (2024). Demodicosis: A clinicopathological study. J Am Acad Dermatol.;60:453-62. Google Scholar
[4]
Pawan Prasher, Dolly Baghra, Drishtant Singh, Anup Kumar Kesavan (2020). Molecular identification and phylogenetic relationship of Demodex mites based on mitochondrial 16S rDNA. Trop Parasitol.; 10(2):136-141. Google Scholar
[5]
Sara Królik, Agnieszka Muth, Dorota Jenerowicz (2020). Demodicosis in humans as a current problem in dermatology and ophthalmology. Journal of Face Aesthetics 2020;3(2):108-121 Google Scholar
[6]
Zhao YE, Wu LP, Hu L, Xu Y, Wang ZH (2012). Sequencing for complete rDNA sequences (18S, ITS1, 5.8S, ITS2, and 28S rDNA) of Demodex and phylogenetic analysis of Acari based on 18S and 28S rDNA. Parasitol Res. 2012;111:2109-14. Google Scholar
[7]
Zhao YE, Ma JX, Hu L, Wu LP, De Rojas M (2013). Discrimination between Demodex folliculorum (Acari: Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences. J Zhejiang Univ Sci B. (14):829-36. Google Scholar