Articles Vol. 65 No. CĐ 12 - Bệnh viện Thủ Đức 12/12/2024

5. EVALUATING THE NRF2 INHIBITORY ACTIVITY OF EXTRACTS FROM THE VIETNAMESE MEDICINAL PLANT HELICTERES HIRSUTA USING A ZEBRAFISH MODEL

Nguyen Thanh Vu1,2, Le Thi Ngoc Tam3,4,5,6, Le Nguyen Thien Han3,4,5,6, Nguyen Kim Anh3,4,5,6, Thai Khac Minh3,4,5,6, Le Minh Tri3,4,5,6, Nguyen Minh Hien3,4,5,6
1 Biotechnology Center of Ho Chi Minh City
2 Trung tâm Công nghệ Sinh học Thành phố Hồ Chí Minh
3 University of Health Sciences, Vietnam National University at Ho Chi Minh City
4 Vietnam National University at Ho Chi Minh City
5 Trường Đại học Khoa học Sức khỏe, Đại học Quốc gia Thành phố Hồ Chí Minh
6 Đại học Quốc gia Thành phố Hồ Chí Minh
Corresponding author: nmhien@uhs.edu.vn
DOI: 10.52163/yhc.v65iCD12.1815
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Keywords
Helicteres hirsuta inhibit Nrf2 Keap1 zebrafish
Abstract

Background: Helicteres hirsuta Lour. is a medicinal plant traditionally used to treat liver diseases.

Aim: This study aimed to evaluate the Nrf2 inhibitory activity of the n-hexane extract from H. hirsuta leaves (AX-He) using a zebrafish model.

Method: Wild-type (AB) and Keap1b knockout (keap1bdl40) zebrafish larvae were treated with AX-He at various concentrations. The nrf2dl703 knockout line was used as a negative control for Nrf2 activity. The antioxidant activity of AX-He was assessed by its ability to protect WT larvae against H2O2-induced oxidative stress. The expression of Nrf2 target genes, including gstp1, prdx1, and nrf2a, was analyzed by RT-qPCR.

Results: AX-He suppressed the expression of prdx1 and nrf2a in keap1bdl40 larvae at concentrations of 50 and 75 µg/mL. At 100 µg/mL, AX-He reduced the antioxidant capacity of WT larvae against H2O2 exposure.

Conclusion: To sum up, this study provides evidence that AX-He can inhibit Nrf2, suggesting the potential use of H. hirsuta in treating diseases associated with Nrf2 dysregulation, including cancer.

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