Articles Vol. 65 No. 6 30/10/2024

30. RESEARCH ON CREATING HUMAN LIVER CANCER TUMORS USING HEP-3B CANCER CELL LINE IN EXPERIMENTS

Nguyen Song Hai1,2, Luu Truong Thanh Hung2,3
1 Military Institude of Traditional Medicine
2 Viện Y học Cổ truyền Quân đội
3 Military Institude of Traditioal Medicine
Corresponding author: nsonghai@gmail.com
DOI: 10.52163/yhc.v65i6.1684
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Keywords
Hep-3B liver cancer Nude mice
Abstract

Objective: To generate human liver cancer tumors in immunodeficient mice (nude mice) using the human liver cancer cell line Hep-3B.

Methods: Cultivation and proliferation of Hep-3B human liver cancer cells, transplantation of 106 Hep-3B cells under the skin of each nude mouse thigh, monitoring mouse condition, formation and development tumor for 35 days. Determine the tumor growth rate, tumor regression rate, tumor volume, survival - death rate, and pathological images of the tumor.

Results: Successfully cultured and proliferated human liver cancer cells of the Hep-3B line, enough cells to serve tumor grafting on nude mice. When transplanting human liver cancer cells from the Hep-3B line, the tumor appearance rate reached 87.5% after 7 days of transplantation, the tumor regression rate was 0%, the average tumor volume after 35 days of transplantation was 774.8 mm3. No mice died due to technical errors and throughout the experiment, the pathological images were similar to human tumors.

Conclusion: Creating an immune-deficient mouse model carrying human liver cancer of the Hep-3B line.

References
[1]
Lê Hoài Thương, Trần Ngọc Ánh, Đậu Quang Liêu, Một số đặc điểm dịch tễ, lâm sàng và cận lâm sàng của bệnh nhân ung thư biểu mô tế bào gan tại Bệnh viện Đại học y Hà Nội, Tạp chí Nghiên cứu y học, 2021, 147 (11), tr 92-100. Google Scholar
[2]
Nguyễn Thị Thu Hường, Đánh giá kết quả điều trị của thuốc sorafenib trên bệnh nhân ung thư gan nguyên phát, Luận án tiến sĩ, Đại học y Hà Nội, 2020, tr 3-5. Google Scholar
[3]
Bùi Khắc Cường, Hồ Anh Sơn, Nguyễn Lĩnh Toàn, Nghiên cứu tạo khối ung thư đại tràng người trên chuột thiếu hụt miễn dịch bằng kỹ thuật ghép dị loài, Tạp chí Y Dược học quân sự, 2012, (9), tr 53-57. Google Scholar
[4]
Siegel RL et al, Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries, CA Cancer J Clin, 2021, 71(3), tr 209-249. Google Scholar
[5]
Giulio Alessandri et al, Single-Shot Local Injection of Microfragmented Fat Tissue Loaded with Paclitaxel Induces Potent Growth Inhibition of Hepatocellular Carcinoma in Nude Mice. Cancers 2021, 13(21), 5505-5512. Google Scholar
[6]
Liu J, Johnston MR, Experimental animal models for studying lung cancers, SurgOncol, 2002, 11(4), 217-27. Google Scholar
[7]
Céspedes MV, Espina C, García-Cabezas MA et al, Orthotopic microinjection of Human Colon Cancer Cells in Nude mice induces tumor foci in all clinically relevant metastics site, Am J Pathol, 2007, 170 (3), 90-97. Google Scholar